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A novel RP‐HPLC refractive index detector method development and validation for determination of trace‐level alcohols (un‐sulfated) in sodium lauryl sulfate raw material
Author(s) -
Katakam Lakshmi Narasimha Rao,
Dongala Thirupathi
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4827
Subject(s) - chemistry , chromatography , high performance liquid chromatography , sodium sulfate , acetonitrile , sodium , sulfation , analytical chemistry (journal) , organic chemistry , biochemistry
The main aim of the study is to develop and validate a simple and rapid liquid chromatographic analytical method for simultaneous determination of trace level of un‐sulfated alcohol impurities in sodium lauryl sulfate using high‐performance liquid chromatography with a refractive index detector. The chromatographic separation was achieved using flow rate of 3.0 ml/min with a Waters Symmetry C 18 (150 × 4.6 mm, 5 μm) column and 50°C as a column temperature. The mobile phase comprised milliQ water and acetonitrile in the ratio of 30:70 v/v respectively. The detection was performed using a refractive index detector at a sensitivity of 64. The resolutions among n‐ decanol, n‐ dodecanol, n‐ tetradecanol, n‐ hexadecanol, and n‐ heptadecanol were found to be >4. Regression analysis showed a correlation coefficient for the stated compounds of >0.999. The validated HPLC method was utilized effectively for the estimation of un ‐ sulfated alcohols in quality control labs for commercial release of sodium lauryl sulfate.