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Spectrum‐effect relationship between fingerprints and hemopoietic effects of small molecular fraction of Polygoni Multiflori radix praeparata
Author(s) -
Wang JunXing,
Fan LuoDi,
Hu MingHua,
Ma FangLi,
Qi Jin
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4821
Subject(s) - emodin , chemistry , radix (gastropod) , haematopoiesis , aplastic anemia , hematocrit , chromatography , hemoglobin , high performance liquid chromatography , biochemistry , immunology , botany , endocrinology , biology , bone marrow , genetics , stem cell
Polygoni multiflori Radix Praeparata (PMRP) is a traditional medicine used for nourishing essence and blood in China. However, it is unclear which PMRP compounds are responsible for its hematopoietic effect. In this study, spectrum‐effect relationship was used to discovery potential hematopoietic compounds. The fingerprints of 20 PMRP batches were established by HPLC and the hematopoietic effect was determined using red blood cell, hemoglobin, hematocrit, and platelet indexes in aplastic anemia model mice. The spectrum‐effect relationship between common peaks and hematopoietic efficacy values was established using gray relational analysis and partial least squares analysis. Spectrum‐effect relationship results showed that peaks 21 (emodin‐8‐ O ‐(6´‐ O ‐acetyl)‐ β ‐D‐glucoside), 15 (2, 3, 5, 4′‐tetrahydroxystilbene‐2‐ O ‐di‐glucoside), 16 (cis‐2,3,5,4′‐tetrahydroxy‐stilbene‐2‐ O ‐ β ‐D‐glucoside), 11 (unknown), 20(unknown, 12 (epicatechin), 29 (carboxyl emodin), and 31 (emodin) in the fingerprints were closely related to the hematopoietic effect. This work successfully established the spectrum‐effect relationship between PMRP hematopoietic effect and its fingerprints, which can be used to explain the material basis for the PMRP hematopoietic effect.