Determining the procedure for routine residue monitoring of sulfamethazine in edible animal tissues

A simplified method to determine/identify residual sulfamethazine (SMZ) in edible tissues from cattle, pigs, chickens and sheep by a high‐performance liquid chromatography (HPLC) with a photo‐diode array detector is presented. The sample preparation was performed by homogenizing with 30% (v/v) ethanol in water followed by an Ultrafree®‐MC/PL as a centrifugal ultra‐filtration unit. For determination/identification of SMZ, a reversed‐phase C 4 column and a mobile phase of 15% (v/v) ethanol in water with a photo‐diode array detector was used. Average recoveries from spiked SMZ (0.1–1.0 mg kg −1 ) were ≥80% with coefficients of variation between 1.3 and 4.3%. The limits of quantitation were calculated to be 0.057–0.060 mg kg −1 . The total time and solvent required for the analysis of one sample were <40 min and <2 mL of ethanol, respectively. Copyright © 2001 John Wiley & Sons, Ltd.