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Analysis of variations in the contents of steroidal saponins in Fructus Tribuli during stir‐frying treatment
Author(s) -
Zhang Chao,
Wang Shuyue,
Guo Fei,
Ma Ting,
Zhang Longfei,
Sun Linlin,
Wang Yingzi,
Zhang Xuelan
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4794
Subject(s) - chemistry , glycoside , chromatography , transformation (genetics) , biotransformation , stereochemistry , organic chemistry , biochemistry , enzyme , gene
Just as natural saponins transform into aglycones, secondary glycosides and their derivatives using biotransformation technology, steroidal saponins may also undergo similar transformation after stir‐frying. The purpose of this study was to elucidate the variations and the reasons for these variations in the contents of steroidal saponins in Fructus Tribuli (FT) during a stir‐frying treatment. Stir‐fried FT was processed in different time–temperature conditions. An UHPLC–MS/MS method was established and fully validated for quantitative analysis. In addition, the simulation processing products of tribuluside A, terrestroside B, terrestrosin K, terrestrosin D and 25 R ‐tribulosin were determined by qualitative analysis using UHPLC–Q‐TOF–MS. The established UHPLC–MS/MS method provides a rapid, flexible, and reliable method for the quality assessment of FT. The present study revealed that furostanol saponins with a C22‐OH group could transform into corresponding furostanol saponins with a C‐20–C‐22 double bond (FSDB) via dehydroxylation. Additionally, FSDB could be successively converted into its secondary glycosides via a deglycosylation reaction. The transformation of spirostanol saponins into corresponding aglycones via deglycosylation led to a decrease in spirostanol saponins and an increase in aglycones. The results of this research provided scientific evidence of variation and structural transformation among steroidal saponins. These findings might be helpful for elucidating the processing mechanism of FT.