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An improved method for proteomics studies in C. elegans by fluorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography–tandem mass spectrometric identification
Author(s) -
Masuda Mayumi,
Saimaru Hiroshi,
Takamura Norio,
Imai Kazuhiro
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.479
Subject(s) - chemistry , chromatography , electrospray ionization , tandem mass spectrometry , proteomics , high performance liquid chromatography , derivatization , mass spectrometry , enzyme , biochemistry , gene
An improved method for proteomics studies, which includes the uorogenic derivertization of protein mixtures with 7‐chloro‐4‐(dimethylaminoethylaminosulfonyl)‐2,1,3‐benzoxadiazole (DAABD‐Cl), followed by HPLC isolation, enzymatic digestion and idetication of the derivatized proteins by HPLC‐electrospray ionization (ESI)‐MS/MS with the probability‐based protein identication algorithm, identied 103 proteins in the soluble extract (10 µg protein) of Caenorhabditis elegans . Copyright © 2005 John Wiley & Sons, Ltd.