An improved method for proteomics studies in  C. elegans  by ﬂuorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography–tandem mass spectrometric identiﬁcation | Zendy

Masuda Mayumi | Zendy; Saimaru Hiroshi | Zendy; Takamura Norio | Zendy; Imai Kazuhiro | Zendy

Premium

An improved method for proteomics studies in C. elegans by ﬂuorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography–tandem mass spectrometric identiﬁcation

Author(s) -

Masuda Mayumi,

Saimaru Hiroshi,

Takamura Norio,

Imai Kazuhiro

Publication year - 2005

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.479

Subject(s) - chemistry , chromatography , electrospray ionization , tandem mass spectrometry , proteomics , high performance liquid chromatography , derivatization , mass spectrometry , enzyme , biochemistry , gene

An improved method for proteomics studies, which includes the uorogenic derivertization of protein mixtures with 7‐chloro‐4‐(dimethylaminoethylaminosulfonyl)‐2,1,3‐benzoxadiazole (DAABD‐Cl), followed by HPLC isolation, enzymatic digestion and idetication of the derivatized proteins by HPLC‐electrospray ionization (ESI)‐MS/MS with the probability‐based protein identication algorithm, identied 103 proteins in the soluble extract (10 µg protein) of Caenorhabditis elegans . Copyright © 2005 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research