Premium
LC–MS/MS analysis of puerarin and 18 β ‐glycyrrhetinic acid in human plasma after oral administration of Samso‐eum and its application to pharmacokinetic study
Author(s) -
Lee Seon Yu,
Jeong Ji Hyun,
Kim Bo Na,
Park So Jung,
Park YangChun,
Lee Guk Yeo
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4774
Subject(s) - chemistry , hesperetin , puerarin , glycyrrhizin , chromatography , pharmacokinetics , naringin , hesperidin , bioavailability , daidzin , oral administration , tandem mass spectrometry , pharmacology , daidzein , mass spectrometry , biochemistry , flavonoid , antioxidant , genistein , medicine , alternative medicine , pathology
The aim of this study was to confirm pharmacokinetic screening of multiple components in healthy Korean subjects after oral administration of Samso‐eum and perform quantitation of active components in the human plasma. Thirteen potential bioactive components [puerarin (PRR), daidzin, nodakenin, ginsenoside Rb1, 18 β ‐glycyrrhetinic acid (18 β ‐GTA), 6‐shogaol, naringin, glycyrrhizin, hesperidin, platycodin D, naringenin, hesperetin, and 6‐gingerol] were screened based on literature. The results showed that three analytes (daidzin, naringenin, and hesperetin) were detected in trace amounts. In addition, PRR and 18 β ‐GTA were detected in human plasma after the oral administration of Samso‐eum. In this study, a liquid chromatography–electrospray ionization‐tandem mass spectrometry method was validated for the simultaneous determination of PRR and 18 β ‐GTA in human plasma. This was the first study to evaluate pharmacokinetics of PRR and 18 β ‐GTA after the usual oral dose of Samso‐eum (30 g containing 102.48 mg PRR, 48.18 mg glycyrrhizin) in human subjects.