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Validation and application of a high‐performance liquid chromatography–tandem mass spectrometry assay for mosapride in human plasma
Author(s) -
Ramakrish. V. S.,
Vishwottam K. N.,
Manoj S.,
Koteshwara M.,
Chidambara J.,
Varma D. P.
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.477
Subject(s) - chromatography , chemistry , analyte , mosapride , mass spectrometry , liquid chromatography–mass spectrometry , detection limit , formic acid , tandem mass spectrometry , selected reaction monitoring , protein precipitation , extraction (chemistry) , electrospray ionization , analytical chemistry (journal) , medicine , pharmacology
A simple, rapid, sensitive and specic liquid chromatography–tandem mass spectrometry method was developed and validated for quantication of mosapride (I), a novel and potent gastroprokinetic agent that enhances the upper gastrointestinal motility by stimulating 5‐HT 4 receptor. The analyte and internal standard, tamsulosin (II), were extracted by liquid–liquid extraction with diethyl ether–dichloromethane (70:30, v/v) using a Glas‐Col Multi‐Pulse Vortexer. The chromatographic separation was performed on a reversed‐phase Waters symmetry C 18 column with a mobile phase of 0.03% formic acid–acetonitrile (10:90, v/v). The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The mass transitions m / z 422.3 → 198.3 and m / z 409.1 → 228.1 were used to measure I and II, respectively. The assay exhibited a linear dynamic range of 0.5–100.0 ng/mL for mosapride in human plasma. The lower limit of quantitation was 500 pg/mL with a relative standard deviation of less than 15%. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges. A run time of 2.0 min for each sample made it possible to analyze a throughput of more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2005 John Wiley & Sons, Ltd.