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Chemical composition and anti‐complement activity of Glechomae Herba collected in different months
Author(s) -
Luo Yun,
Wen Quan,
Yang Shilin,
Feng Yulin,
Tan Ting
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4762
Subject(s) - chemistry , chemical composition , composition (language) , complement (music) , complement system , chlorogenic acid , chromatography , food science , biochemistry , organic chemistry , antibody , philosophy , linguistics , complementation , biology , immunology , gene , phenotype
Abstract Glechomae Herba (GH) is derived from the dried aerial part of Glechoma longituba (Nakai) Kupr., which is harvested from spring to autumn. It has the effects of clearing heat and detoxification. The aim of this paper was to study the chemical composition and the anti‐complement activity of GH collected in different months. Ultra‐high‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry based on predicted compounds screening and diagnostic ion filter strategy was developed for identifying the chemical composition of GH collected in different months. A total of 102 compounds—40 chlorogenic acids (CGAs), 32 phenolic acids, and 30 flavonoids—were reasonably identified in GH. Thirty‐four CGAs were discovered in GH for the first time. The correlations between chemical compositions and anti‐complement activities of GH collected in different months were analyzed. Phenolic acids and flavonoids were found to be negatively correlated with anti‐complement activity, and CGAs were positively correlated with anti‐complement activity. At the same time, six CGA standards had obvious anti‐complement activity. It was demonstrated that different harvest months had a significant impact on the difference in chemical composition and anti‐complement activity of GH. And CGAs might play an important role in the anti‐complement activity of GH.