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Role of anti‐inflammatory interventions in high‐fat‐diet‐induced obesity
Author(s) -
Ghazala Rasha Abdelmawla,
El Medney Azza,
Meleis Anisa,
Mohie El dien Passant,
Samir Hend
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4743
Subject(s) - safranal , celecoxib , chemistry , lipotoxicity , arachidonic acid , eicosapentaenoic acid , medicine , endocrinology , fatty acid , pharmacology , obesity , biochemistry , polyunsaturated fatty acid , enzyme , insulin resistance , crocin
Abstract Lipotoxicity is defined as deposition of excess fat associated with an inflammatory response. Metabolomic analysis of fatty acids (FAs) can be a marker of silent inflammation. ω3‐Enriched diet, celecoxib, and safranal may have a protective anti‐inflammatory role. In this work, total FAs extracted from red blood cells and arachidonic acid‐to‐eicosapentaenoic acid (AA‐to‐EPA) ratios were assessed using GC–MS assay in single‐ion monitoring mode. The study was conducted on 64 male rats divided into eight groups: I, controls; II, rats received high‐fat diet (HFD), III, rats received ω‐6‐enriched HFD; IV, rats received ω‐3‐enriched HFD; V, rats received celecoxib with HFD; VI, rats received safranal with HFD; VII and VIII, rats received celecoxib and safranal with ω‐3 HFD, respectively. GC–MS Gas chromatography Mass spectrometry was performed for analysis of fatty acid methyl ester. Enzyme‐linked immunosorbent assay was used to analyze serum interleukin‐6 (IL‐6) and transforming growth factor‐beta 1 (TGF‐β1) concentrations. A statistically significant decrease of AA‐to‐EPA ratio was observed in group VII when compared with the groups receiving HFDs. This group also showed the lowest serum IL‐6 level and highest TGF‐β1 level. In conclusion, ω3‐enriched diet along with drugs (e.g. celecoxib) and herbal medications (e.g. safranal) may have an anti‐inflammatory effect in lipotoxicity. GC–MS with single‐ion monitoring is valid for the analysis of FAs.