Premium
An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody
Author(s) -
Kitisripanya Tharita,
Sermpradit Witcha,
Sakamoto Seiichi,
Tanaka Hiroyuki,
Putalun Waraporn
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4718
Subject(s) - artesunate , chromatography , immunoassay , monoclonal antibody , chemistry , calibration curve , standard curve , antibody , detection limit , plasmodium falciparum , malaria , biology , immunology
There have been reports of fake artesunate (ART), which has led to deaths from untreated malaria in South East Asia. To rapidly screen for fake and adulterated ART products in the drug market, a lateral flow immunoassay (LFIA) based on a colloidal gold–monoclonal antibody probe for detection of ART within samples was developed. With this method, the calibration curve for ART was determined by the intensity ratio of the test and control bands at various ART concentrations. The linearity range was 12.5–200 μg/ml of ART. Samples were tested by the developed LFIA and can be calculated for ART contents. The levels of ART in the samples were also confirmed by enzyme‐linked immunosorbent assay. The results of the two methods were in good conformance. The proposed LFIA was demonstrated to be a simple and rapid analytical method for detecting ART in the pharmaceutical formulation.