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Validation and application of a novel UHPLC–MS/MS method for the measurement of furanodienone in rat plasma
Author(s) -
Deng Zhipeng,
Liu Qian,
Wu Weihong,
Wang Hong
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4717
Subject(s) - chemistry , chromatography , pharmacokinetics , plasma , tandem mass spectrometry , mass spectrometry , patchouli , pharmacology , medicine , physics , quantum mechanics , essential oil
A sensitive ultra‐high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was established to analyze furanodienone in rat plasma. In the process of chromatographic separation, selected reaction monitoring transitions for furanodienone and patchouli alcohol (internal standard, IS) were m/z 231.1 → 83.2 and m/z 205.1 → 95.1, respectively. Great linearity of furanodienone in plasma samples was found in the corresponding concentration range ( r > 0.995). Intra‐ and inter‐day precisions (RSD, %) were <11.3% in plasma, and the accuracy (RE, %) was within ±10.7%. This method was used to the furanodienone study on rat pharmacokinetics after a single oral dose of 10 mg/kg of furanodiene. The results indicated that the maximum observed plasma concentration was 52.4 ± 19.1 ng/ml at 1.2 ± 0.7 h with an elimination half‐life of 2.2 ± 0.7 h. The obtained data indicated that furanodienone could be moderately distributed and eliminated.