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A novel approach to the quantification of urinary aryl‐propionamide‐derived SARMs by UHPLC–MS/MS
Author(s) -
Temerdashev Azamat,
Dmitrieva Ekaterina,
Azaryan Alice,
Gashimova Elina
Publication year - 2020
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4700
Subject(s) - chemistry , chromatography , chloroform , extraction (chemistry) , analyte , calibration curve , detection limit , urine , methanol , linear range , organic chemistry , biochemistry
A simple and sensitive procedure for the quantification of two commonly abused aryl‐propionamide‐derived selective androgen receptor modulators (SARMs), namely S‐4 (GTx‐007, andarine) and S‐22 (GTx‐024, MK‐2866, ostarine, enobosarm), has been described. Urine samples were prepared for analysis by means of a dispersive liquid–liquid microextraction using methanol and chloroform as dispersive and extracting solvents, respectively. Factors that might influence the extraction process as well as their optimum conditions were evaluated by Box–Benken and central composite designs. After extraction, the analytes were quantified by UHPLC–MS/MS. The proposed procedure was validated on human urine samples. As a result, for both SARMs the detection limits were observed at 0.05 ng/mL and calibration curves were linear in the concentration range of 0.25–50 ng/mL with the coefficient of determination of 0.998.