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Construction of molecularly imprinted nanoparticles by employing ultrasound waves for selective determination of doxepin from human plasma samples: Modeling and optimization
Author(s) -
Bahrani Sonia,
Ghaedi Mehrorang,
Arabi Maryam
Publication year - 2019
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4675
Subject(s) - ethylene glycol dimethacrylate , chemistry , detection limit , chromatography , sonication , methacrylic acid , precipitation polymerization , solid phase extraction , sorbent , column chromatography , doxepin , central composite design , fourier transform infrared spectroscopy , polymerization , adsorption , radical polymerization , response surface methodology , chemical engineering , organic chemistry , medicine , engineering , pharmacology , polymer
Abstract In this work, molecularly imprinted nanoparticles (MINPs) were applied as selective adsorbent for ultrasound‐assisted micro‐solid‐phase extraction (UAMSPE) of doxepin (DP) from human plasma samples, which was then cleaned up, pre‐concentrated and subjected to HPLC. The MINPs were synthesized based on a non‐covalent approach by precipitation polymerization utilizing methacrylic acid and styrene as functional monomers, DP as template, ethylene glycol dimethacrylate as cross‐linker and 2,2‐azobisisobutyronitrile (AIBN) as initiator. The obtained MINPs were characterized by Fourier transform‐infrared and field emission scanning electron microscopy. Factors influencing the efficiency of UAMSPE such as sonication time, volume of eluent solvent and amount of sorbent were investigated using a central composite design and the optimal points were identified as 4 min of sonication time, 380 μL of eluent solvent and 30 mg of sorbent. Under optimized conditions, the proposed method has linear responses in the range of 0.2–2000 ng mL –1 , with a satisfactory limit of detection of 0.04 ng mL –1 and limit of quantification of 0.11 ng mL –1 .