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Validation and application of a novel LC/MS/MS method for the determination of isoginkgetin in rat plasma
Author(s) -
Zhao Cuimei,
Han Song,
Yang Shengbao,
Xin Weidong
Publication year - 2019
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4667
Subject(s) - chemistry , chromatography , extraction (chemistry) , liquid chromatography–mass spectrometry , accuracy and precision , mass spectrometry , tandem mass spectrometry , matrix (chemical analysis) , pharmacokinetics , pharmacology , medicine , statistics , mathematics
Isoginkgetin is a biflavonoid compound isolated from the leaf extracts of Ginkgo biloba . In this study, an liquid chromatography–tandem mass spectrometry (LC/MS/MS) with liquid–liquid extraction was developed and validated for the analysis of isoginkgetin in rat plasma. In the process of chromatographic separation, selected reaction monitoring transitions for isoginkgetin and IS were m/z 566.8 → 134.7 and m/z 430.8 → 269.3, respectively. The validation parameters including selectivity, linearity, LLOQ, accuracy, precision, matrix effect, stability and recovery were satisfactory. The intra‐ and inter‐batch precision (RSD) were <12.1% in plasma, while the accuracy (RE) was within ±14.3%. This method was employed in a pharmacokinetic study on rats after the intravenous administration of isoginkgetin.