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Determination and tissue distribution studies of dantrolene sodium with hydroxypropyl‐ β ‐cyclodextrin in rat tissue by HPLC/MS/MS
Author(s) -
Liu Ruixue,
Chen Yu,
Zhao Shen,
Zhao Min,
Zhao Chunjie,
Wang Miao
Publication year - 2019
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4616
Subject(s) - chemistry , chromatography , high performance liquid chromatography , bioavailability , electrospray ionization , dantrolene sodium , protein precipitation , pharmacokinetics , quantitative analysis (chemistry) , dantrolene , sodium , mass spectrometry , methanol , tissue distribution , pharmacology , medicine , organic chemistry , calcium
The established analytical method for determining the concentration of dantrolene sodium (Da) in rat tissues by HPLC/MS/MS technique was successfully applied to tissue distribution studies of Da in rats. Tissue homogenate samples were pretreated by protein precipitation with pre‐cooled methanol. Chromatographic separation was achieved on an Acquity HPLC column (Kromat Universil XB‐C 18 , 2.1 × 150 mm, 3 μm). Mass spectrometry was conducted with an electrospray ionization interface in negative ionization mode and multiple reaction monitoring was used for quantitative analysis. The results showed that Da was rapidly and widely distributed in tissues and reached the maximum concentration within 0.5 h in all tissues after oral administration of Da–hydroxypropyl‐ β ‐cyclodextrin (DHC). It was then metabolized by liver and finally excreted from kidney,which indicated that DHC inclusion complex has better absorption and higher oral bioavailability than Da. The results also provided evidence for the safety and effectiveness of drug clinical application.