Premium
Study on the bioavailability of puerarin from Pueraria lobata isoflavone self‐microemulsifying drug‐delivery systems and tablets in rabbits by liquid chromatography–mass spectrometry
Author(s) -
Cui Shengmiao,
Zhao Chunshun,
Tang Xing,
Chen Dawei,
He Zhonggui
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.460
Subject(s) - puerarin , pueraria , chemistry , chromatography , bioavailability , analyte , pharmacokinetics , electrospray ionization , detection limit , mass spectrometry , pharmacology , medicine , alternative medicine , pathology
To evaluate the bioavailability of puerarin from Pueraria lobata isoavone self‐microemulsifying drug delivery systems (SMEDDS) and Yufengningxin tablets, a rapid and specic liquid chromatography–mass spectrometric method was developed and validated to determine puerarin in rabbit serum. The analyte was extracted from serum samples by precipitating the serum proteins, separated on a Diamonsil C 18 column and detected by mass spectrometry with an electrospray ionization interface. 4‐Hydroxybenzaldehyde was used as the internal standard. The method has a limit of quantitation of 10 ng/mL using 200 µL serum. The intra‐day relative standard deviations (RSDs) ranged from 3.7 to 6.9% and inter‐day RSDs were within 6.5%. After administration of SMEDDS and tablets to rabbits, a signicant difference was observed in main pharmacokinetic parameters of t max , C max and AUC 0−∞ between SMEDDS and tablets, and a 2.2‐fold increase in the relative bioavailability of puerarin was observed with the SMEDDS compared with Yufengningxin tablets. It was concluded that the absorption of puerarin from Pueraria lobata isoavone SMEDDS was enhanced. Copyright © 2004 John Wiley & Sons, Ltd.