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Therapeutic monitoring of imipramine and desipramine by micellar liquid chromatography with direct injection and electrochemical detection
Author(s) -
Bose Devasish,
MartinavarroDomínguez Adrià,
GilAgustí Mayte,
CardaBroch Samuel,
Durgbanshi Abhilasha,
CapellaPeiró MariaElisa,
EsteveRomero Josep
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.455
Subject(s) - chromatography , chemistry , desipramine , imipramine , metabolite , calibration curve , micellar liquid chromatography , active metabolite , detection limit , therapeutic drug monitoring , quantitative analysis (chemistry) , pharmacokinetics , micelle , pharmacology , biochemistry , medicine , alternative medicine , pathology , aqueous solution , hippocampus , antidepressant
A micellar liquid chromatographic (MLC) procedure was developed for the clinical monitoring of imipramine and its active metabolite, desipramine. The determination of these highly hydrophobic substances was carried out after direct injection of the serum samples using a mobile phase composed of 0.15 m SDS–6% (v/v) pentanol buffered at pH 7, pumped at 1.5 mL/min into a C 18 column (250 × 4.6 mm), and electrochemical detection at 650 mV. Using this MLC method, calibration was linear ( r > 0.995) and the limits of detection (ng/mL) were 0.34 and 0.24 for imipramine and desipramine, respectively. Repeatabilities and intermediate precision were tested at three different concentrations in the calibration range and a CV (%) below 2.2 was obtained. In this MLC procedure, the serum is determined without treatment, thus allowing repeated serial injections without changes in retention factors, and reducing the time and consumables required to carry out the pretreatment process. The assay method can be applied to the routine determination of serum imipramine and its metabolite in therapeutic drug monitoring. Copyright © 2004 John Wiley & Sons, Ltd.