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Simultaneous quantification of six ephedrines in a Mahwang preparation and in urine by high‐performance liquid chromatography
Author(s) -
Chan Kuei Hui,
Pan RyhNan,
Hsu MeiChich
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.452
Subject(s) - chromatography , chemistry , analyte , ephedrine , pseudoephedrine , urine , elution , detection limit , sample preparation , biochemistry , neuroscience , biology
A rapid and reliable high‐performance liquid chromatographic method was developed and validated for the simultaneous determination of norephedrine (NE), norpseudoephedrine (NPE), ephedrine (E), pseudoephedrine (PE), methylephedrine (ME) and methylpseudoephedrine (MPE) in both a Mahwang traditional Chinese medicine (TCM) preparation and in urine using α ‐ethylbenzylamine as the internal standard. The method uses a Spherisorb C 18 column for an isocratic elution in a tetraethylammoniumphosphate‐methanol mobile phase at a wavelength of 206 nm. The limits of detection of NE, NPE, E, PE, ME and MPE in sample solutions ranged from 0.1 to 0.3 µg[sol ]mL at a signal‐to‐noise ratio of 3. The within‐day precision as calculated from the Mahwang TCM preparation and urine samples was below 6.2 and 1.4% for each analyte. The between‐day precision as calculated from the Mahwang TCM preparation and urine samples was below 6.8 and 5.9% for each analyte. The between‐day accuracy as determined from the Mahwang TCM preparation and urine samples was below 2.2 and 6.8% for each analyte. The recoveries for six compounds, obtained with compounds spiked into the Mahwang TCM preparation and urine, were found to be more than 93.6%. This method can be successfully applied to doping and excretion rate studies. Copyright © 2005 John Wiley & Sons, Ltd.