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Determination of olanzapine for therapeutic drug monitoring in schizophrenia patients by LC/MS method
Author(s) -
Albayrak Mevlut,
Kadioglu Yucel,
Yaman Mehmet Emrah,
Senol Onur,
Oral Elif
Publication year - 2019
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4468
Subject(s) - chemistry , chromatography , olanzapine , calibration curve , diethyl ether , acetic acid , therapeutic drug monitoring , extraction (chemistry) , drug , detection limit , pharmacology , schizophrenia (object oriented programming) , organic chemistry , medicine , computer science , programming language
Olanzapine is an atypical antipsychotic drug from the thienobenzodiazepine family which displays efficacy in patients with schizophrenia and related psychoses. A novel LC/MS method was developed and validated for determination of olanzapine in schizophrenia patients' plasma. A liquid–liquid extraction procedure was carried out using 5 mL diethyl ether–diisopropyl ether mixture (1:1, v/v). Average recovery of the extraction procedure was 94.8%. Chromatographic separation was performed on reversed‐phase C 18 column (250 × 2.0 mm, 5 μm) using mixture of deionized water (trifluoro acetic acid 0.1%)–acetonitrile (20:80, v/v) as mobile phase at a flow rate of 1 mL/min. Irbesartan was used as internal standart and total run time was 2.5 min. Mass spectrometric analysis were carried out in selective‐ion montoring mode, and detected olanzapine at m / z 313.1 and IS at m / z 429.4 in all forms of the ions. The calibration curve of olanzapine was linear in the range 2–300 ng/mL ( r 2 > 0.9993). The interday and intraday precisions (RSD) were <7.55%, and accuracy was >7.59% ( n = 6). The proposed study was successfully validated with respect to the US Food and Drug Administration guidelines.