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Meropenem and ciprofloxacin in complicated gastric surgery for cancer patients: A simple SPE–UHPLC–PDA method for their determination in human plasma
Author(s) -
Ferrone Vincenzo,
Cotellese Roberto,
Cichella Annadomenica,
Raimondi Paolo,
Carlucci Maura,
Palumbo Paola,
Carlucci Giuseppe
Publication year - 2019
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4450
Subject(s) - chromatography , chemistry , meropenem , ammonium acetate , ciprofloxacin , detection limit , analyte , resolution (logic) , extraction (chemistry) , elution , sample preparation , solid phase extraction , high performance liquid chromatography , antibiotics , biochemistry , antibiotic resistance , artificial intelligence , computer science
A simple and rapid ultra‐high‐performance liquid chromatographic (UHPLC) for the simultaneous determination of meropenem and ciprofloxacin in human plasma was developed and validated. All of the analytes were separated in <5 min. A solid‐phase extraction method was applied from sample preparation. Analytical separation was performed on a Poroshell SB C 18 column (50 × 2.1 mm, 2.7 μm particle size) with photodiode array (PDA) detection. Meropenem and ciprofloxacin were determined at wavelengths of 300 and 277 nm, respectively. The mobile phase was a mixture of acetonitrile–10 m m ammonium acetate–methanol in gradient elution. The method has been validated for both drugs in gastric surgery for cancer patients. The method showed good linearity with correlation coefficients, r 2 = 0.994 for the two drugs, as well as high precision (RSD < 10.5% in each case); accuracy ranged from −5.8 to +6.0%. The limit of quantitation of the two drugs was established at 0.02 and 0.01 μg/mL, respectively. Meropenem, ciprofloxacin and the internal standard were extracted from human plasma with a mean recovery ranging from 92.5 to 98.6%. The method was applied to quantify the drugs dosage in complicated gastric surgery patients.