Simultaneous determination of tryptophan and kynurenine in serum by HPLC with UV and ﬂuorescence detection | Zendy

Vignau J. | Zendy; Jacquemont M.C. | Zendy; Lefort A. | Zendy; Imbenotte M. | Zendy; Lhermitte M. | Zendy

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Simultaneous determination of tryptophan and kynurenine in serum by HPLC with UV and ﬂuorescence detection

Author(s) -

Vignau J.,

Jacquemont M.C.,

Lefort A.,

Imbenotte M.,

Lhermitte M.

Publication year - 2004

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.445

Subject(s) - kynurenine , chemistry , repeatability , chromatography , indoleamine 2,3 dioxygenase , tryptophan , high performance liquid chromatography , reproducibility , detection limit , biochemistry , amino acid

Tryptophan metabolism is disturbed in mental depression, and the induction of indoleamine 2,3‐dioxygenase increases kynurenine production. In order to determine this disturbance in patients with chronic hepatitis C and receiving interferon‐based immunotherapy, a new and specic HPLC protocol was elaborated. For tryptophan, the assay was linear from 6.25 to 100 µmol L −1 , and the limits of detection (LOD) and quantitation (LOQ) for the method were 0.7 and 8.0 µmol L −1 . For kynurenine, the linearity of calibration was from 0.0625 to 6.25 µmol L −1 , with LOD and LOQ of 2 and 3 nmol L −1 . Reproducibility and repeatability were satisfactory. The method allowed study of human blood serum. Copyright © 2004 John Wiley & Sons, Ltd.

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