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Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC–MS/MS assay
Author(s) -
Niu Kai,
Yan Huiyu,
Guo Chunjie,
Zhang Sixi,
Zhu Wei,
Teng Shiyong
Publication year - 2019
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4417
Subject(s) - chemistry , chromatography , pharmacokinetics , analyte , electrospray ionization , protein precipitation , selected reaction monitoring , centrifugation , mass spectrometry , electrospray , distribution (mathematics) , tandem mass spectrometry , pharmacology , medicine , mathematical analysis , mathematics
A highly sensitive LC–MS/MS method was developed to measure oroxin B in rat plasma and tissue homogenates. The analyte and IS were isolated from biological matrices by a simple protein precipitation followed by centrifugation. Detection was conducted by electrospray negative‐ionization mass spectrometry in selected‐reaction monitoring mode. The assay was linear in the concentration range 4.52–904 ng/mL with intra‐ and inter‐day precision of <14.41%. It was successfully applied to the pharmacokinetics and tissue distribution studies of oroxin B after an intravenous dose of 1.0 mg/kg in rats. The results would be useful for further development of oroxin B.