Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC–MS/MS assay | Zendy

Niu Kai | Zendy; Yan Huiyu | Zendy; Guo Chunjie | Zendy; Zhang Sixi | Zendy; Zhu Wei | Zendy; Teng Shiyong | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC–MS/MS assay

Author(s) -

Niu Kai,

Yan Huiyu,

Guo Chunjie,

Zhang Sixi,

Zhu Wei,

Teng Shiyong

Publication year - 2019

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.4417

Subject(s) - chemistry , chromatography , pharmacokinetics , analyte , electrospray ionization , protein precipitation , selected reaction monitoring , centrifugation , mass spectrometry , electrospray , distribution (mathematics) , tandem mass spectrometry , pharmacology , medicine , mathematical analysis , mathematics

A highly sensitive LC–MS/MS method was developed to measure oroxin B in rat plasma and tissue homogenates. The analyte and IS were isolated from biological matrices by a simple protein precipitation followed by centrifugation. Detection was conducted by electrospray negative‐ionization mass spectrometry in selected‐reaction monitoring mode. The assay was linear in the concentration range 4.52–904 ng/mL with intra‐ and inter‐day precision of <14.41%. It was successfully applied to the pharmacokinetics and tissue distribution studies of oroxin B after an intravenous dose of 1.0 mg/kg in rats. The results would be useful for further development of oroxin B.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research