Premium
Use of liquid chromatography–tandem mass spectrometry for the analysis of pentoxifylline and lisofylline in plasma
Author(s) -
Kyle Patrick B.,
Adcock Kim G.,
Kramer Robert E.,
Baker Rodney C.
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.441
Subject(s) - chemistry , chromatography , mass spectrometry , tandem mass spectrometry , high performance liquid chromatography , analyte , selected reaction monitoring , pentoxifylline , electrospray , analytical chemistry (journal) , medicine , pharmacology
A method was developed for the quantitation of pentoxifylline [1‐(5‐oxohexyl)‐3,7‐dimethylxanthine] and a primary active metabolite, lisofylline [1‐(5‐hydroxyhexyl)‐3,7‐dimethylxanthine], using high‐performance liquid chromatography (HPLC)–tandem mass spectrometry. This method was developed in order to overcome problems encountered with HPLC–ultraviolet detection. The operating parameters of the electrospray interface (PE SCIEX, TurboIon Spray) and lens voltages of the triple‐quadrupole detector (PE SCIEX 365) were optimized in positive ion mode to obtain the best sensitivity of the analytes. Collision‐induced dissociation was used to produce fragment ions, and multiple reaction monitoring was used to quantitate pentoxifylline ( m / z 279/181) and lisofylline ( m / z 263/181). Dichloromethane was used to extract the drug, metabolite, and the internal standard (3‐isobutyl‐1‐methylxanthine) from plasma. A reverse‐phase C8(2) 150 × 1.0 mm HPLC column was used to resolve all three compounds in less than 6 min. Calibration curves were generated using peak area and were linear from 1 to 1000 ng/mL ( R 2 > 0.99). The small sample volume, ease of extraction, and sensitivity provide advantages over more conventional methods of quantitation. Copyright © 2004 John Wiley & Sons, Ltd.