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UPLC–MS/MS method validation of ciprofloxacin in human urine: Application to biodegradability study in microbial fuel cell
Author(s) -
Sharma Priya,
Kumar Devendra,
Mutnuri Srikanth
Publication year - 2019
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4392
Subject(s) - chromatography , chemistry , ciprofloxacin , detection limit , ammonium acetate , urine , elution , mass spectrometry , high performance liquid chromatography , tandem mass spectrometry , biochemistry , antibiotics
To enable the reliable quantification of ciprofloxacin in human urine, a sensitive and selective assay based on liquid chromatography–tandem mass spectrometry was developed. The chromatographic separation of the ciprofloxacin was carried out on a Zorbex Eclipse C 18 column using methanol and ammonium acetate as a mobile phase by the gradient elution method. The developed assay covered a wide range of concentrations (1.56–100 ng/mL) with a lower limit of detection of 0.76 ng/mL. Quantification was performed using the multiple reaction monitoring transitions 331.8/231 for ciprofloxacin and 362/318 for ofloxacin (internal standard). This assay was validated for linearity, accuracy, precision and recovery. The validated method was then applied to the biodegradability of ciprofloxacin (99%) from human urine in the microbial fuel cell.