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Simultaneous ultraperformance liquid chromatography/tandem mass spectrometry determination of four antihypertensive drugs in human plasma using hydrophilic–lipophilic balanced reversed‐phase sorbents sample preparation protocol
Author(s) -
Mahrouse Marianne A.
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4362
Subject(s) - chemistry , chromatography , hydrochlorothiazide , captopril , fosinopril , benazepril , solid phase extraction , liquid chromatography–mass spectrometry , selected reaction monitoring , tandem mass spectrometry , mass spectrometry , angiotensin converting enzyme , blood pressure , medicine , radiology
Therapeutic drug monitoring of angiotensin‐converting enzyme inhibitors has a great impact on blood pressure control in patients with heart failure and hepatic and renal impairment. To provide an efficient tool for drug assessment in plasma, a UPLC–MS/MS method was developed for simultaneous determination of benazepril hydrochloride, fosinopril sodium, captopril and hydrochlorothiazide in human plasma samples. Solid phase extraction was applied for sample preparation using OASIS ® hydrophilic–lipophilic balanced reversed‐phase sorbents cartridges. Chromatographic separation was performed using an Agilent SB‐C 18 column and methanol–0.1% formic acid in water (95:5, v/v) as mobile phase, at flow rate 0.3 mL/min. Detection was accomplished using a tandem mass spectrometer. The method was validated according to US Food and Drug Administration guidelines. It showed good linearity over concentration ranges 5–400 ng/mL for benazepril hydrochloride, fosinopril sodium and hydrochlorothiazide and 100–3500 ng/mL for captopril. CV% values were <13.92% whereas the mean accuracy ranged from 94.50 to 113.82% for quality control samples and their extraction recoveries ranged from 90.60 to 99.38%. In conclusion, the present study revealed method selectivity and sensitivity; it can be applied for estimation of angiotensin converting enzyme inhibitors and hydrochlorothiazide in human plasma for dose adjustment and therapeutic drug monitoring.