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Determination of trehalose by ion chromatography and its application to a pharmacokinetic study in rats after intramuscular injection
Author(s) -
Chen Hui,
Chen Weizhu,
Hong Bihong,
Zhang Yiping,
Hong Zhuan,
Yi Ruizao
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4355
Subject(s) - chemistry , chromatography , pharmacokinetics , standard curve , column chromatography , ion chromatography , high performance liquid chromatography , pharmacology , medicine
An ion chromatography method was established for detecting trehalose in rat plasma. The samples were analyzed using a CPMA1 column (250 × 4.0 mm, Thermo) with 120 m m NaOH as eluent at a flow rate of 0.7 mL/min. The standard curve was y = 1.4316 x − 0.0654 ( R = 0.9992), and the linear range was 0.2–10 mg/L. The relative standard deviations of within‐run and between‐run precisions at low, medium and high concentrations were within 0.96–8.33%, and the accuracy was within 80.09–114.99%. The method was verified by rigorous methods, and applied to a pharmacokinetic study in rats after intramuscular injection (20 mg/kg, n = 6). The pharmacokinetic parameters, specifically AUC 0– t , AUC 0–∞ , t 1/2 , C L and V d , were 15.542 ± 3.122 mg h/L, 15.599 ± 3.141 mg h/L, 0.73 ± 0.347 h, 1.331 ± 0.293 L/h kg and 1.403 ± 0.735 L/kg, respectively. The developed ion chromatography method met the requirements of biological sample measurement, and will be helpful for future pharmacological studies of trehalose.