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A metabolomics research based on UHPLC‐ESI‐Q‐TOF‐MS coupled with metabolic pathway analysis: Treatment effects of stir‐frying Xanthii Fructus on allergic rhinitis in mice model
Author(s) -
Zhuang Yanshuang,
Qin Kunming,
Yu Beibei,
Liu Xiao,
Cai Baochang,
Cai Hao
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4352
Subject(s) - metabolomics , chemistry , metabolic pathway , electrospray ionization , chromatography , mechanism (biology) , time of flight mass spectrometry , mass spectrometry , traditional medicine , metabolism , biochemistry , medicine , ion , philosophy , organic chemistry , epistemology , ionization
Xanthii Fructus (XF), a well‐known herb in traditional Chinese medicine, has been frequently used for the treatment of allergic rhinitis in the clinic. Its therapeutic metabolic mechanism, however, remains undetermined. In this work, a metabolomics research coupled with metabolic pathway analysis has been employed to screen out the potential mechanism in its effects on allergic rhinitis. Specifically, mouse serum samples containing XF were analyzed based on ultra‐high performance liquid chromatography equipped with electrospray ionization quadruple time‐of‐flight mass spectrometry detection (UHPLC‐ESI‐Q‐TOF‐MS) in both positive and negative polarity. In addition, the raw data gained from UHPLC‐ESI‐Q‐TOF‐MS were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS‐DA) in order to discover remarkable metabolites. Twenty‐seven potential biomarkers in mouse serum were filtered from free databases like HMDB. Interestingly, this study filtered the potential metabolic pathways including glycerophospholipid metabolism and branch‐chain amino acid metabolism. We hope that this paper will provide a feasible strategy for revealing the therapeutic mechanism of XF in allergic rhinitis mice model.