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Simultaneous determination of nine analytes related to the pathogenesis of diabetic encephalopathy in diabetic rat cortex and hippocampus by HPLC‐FLD
Author(s) -
Huang Dan,
Jiang QingSong,
Yang JunQing,
Cui Ting,
Wang NianRu,
Du TingTing,
Jiang XinHui
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4338
Subject(s) - chemistry , monoamine neurotransmitter , taurine , neurotransmitter , chromatography , high performance liquid chromatography , serotonin , glutamate receptor , dopamine , hippocampus , glycine , tryptophan , amino acid , medicine , endocrinology , biochemistry , receptor
The determination of amino acids and monoamine with actions like neurotransmitters or modulators has become increasingly important for studying the relationship between the dysfunction of neurotransmitters and the pathogenesis of diabetic encephalopathy. Here, a high‐performance liquid chromatography with fluorescence detection method was developed to simultaneously determine nine monoamines and amino acids including three excitatory neurotransmitters (aspartate, glutamate, and serotonin), four inhibitory neurotransmitters (glycine, γ ‐aminobutyric acid, taurine, dopamine), a precursor of 5‐HT (tryptophan) and methionine using homoserine as the internal standard. The separation was performed on a BDS column with methanol‐buffer solution of 35 mmol/L sodium acetate and 5 mmol/L citric acid (pH 6.0) using a simple gradient elution. Several parameters including specificity, precision, and recovery were validated after optimization of the analytical conditions. The developed method was successfully applied to determine the cortex and the hippocampus samples from Sprague–Dawley rats. Our results showed that various neurotransmitters involved in diabetes mellitus may tend to be differentially modulated and present a different alteration tendency at different time course, which might be associated with the duration of diabetes mellitus.