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Pharmacokinetics of Sijunzi decoction in rat plasma after oral administration using ultra‐high‐performance liquid chromatography electrospray ionization quadrupole–time of flight mass spectrometry
Author(s) -
Qu Gonglin,
Qi Xuesong,
Shao Shuai,
Li Chen,
Wang Chunyan,
Wang Chengfang,
Gou Qiao
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4334
Subject(s) - chemistry , chromatography , formic acid , electrospray ionization , mass spectrometry , decoction , pharmacokinetics , quadrupole time of flight , ginsenoside , electrospray , analytical chemistry (journal) , ginseng , medicine , alternative medicine , pathology , traditional medicine
Sijunzi decoction (SJZD) is one of the most well‐known traditional Chinese herbal formulations. This study elucidates the pharmacokinetics of SJZD in rat plasma after the administration of a single oral dose of 3 mL/kg using ultra‐high‐performance liquid chromatography electrospray ionization quadrupole–time of flight mass spectrometry (UHPLC‐ESI‐Q‐TOF/MS) with bergapten as an internal standard. The separation was performed on an Agilent Zorbax Eclipse Plus C 18 column (2.1 × 50 mm, 1.8 μm) by elution with acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.3 mL/min. Electrospray ionization in positive and negative ion modes was used to quantify six compounds, with monitored ion m/z values of 249.1397 [M + H] + and 529.3857 [M + H] + for atractylenolide III (ATL‐III) and pachymic acid (PA), respectively, and m/z of 1107.6638 [M − H] − , 991.5746 [M – H + HCOO] − , 821.3714 [M − H] − , 469.3315 [M − H] − for ginsenoside Rb 1, Re, glycyrrhizic acid (GL), and glycyrrhetinic acid (GA), respectively. The calibration curves for ginsenoside Rb 1 , Re, ATL‐III, PA, GL and GA were 0.0015–0.75, 0.001–0.5, 0.0004–0.2, 0.003–0.9, 0.0015–0.3 and 0.001–1.5 μg/mL, respectively. The intra‐ and inter‐day precisions (RSD) were <14.3%. The rapid, sensitive and specific UHPLC‐ESI‐Q‐TOF/MS method developed and validated in this study was successfully applied to the simultaneous determination of the six components of SJZD using rat plasma for pharmacokinetic studies after oral administration.