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Analysis of antioxidant compounds in sweet orange peel by HPLC–diode array detection–electrospray ionization mass spectrometry
Author(s) -
Anagnostopoulou Maria A.,
Kefalas Panagiotis,
Kokkalou Eugenios,
Assimopoulou Andrea.,
Papageorgiou Vassilios P.
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.430
Subject(s) - flavones , chemistry , flavonols , chromatography , orange (colour) , electrospray ionization , citrus × sinensis , flavonoid , mass spectrometry , ethyl acetate , high performance liquid chromatography , antioxidant , organic chemistry , food science
HPLC–diode array detection–electrospray ionization mass spectrometry was used to determine qualitatively and quantitatively the avonoid content of several fractions and residues of extracts of Greek navel sweet orange peel ( Citrus sinensis ) from the region of southern Greece (Leonidi‐Tripoli). The main groups of avonoids found according to HPLC retention times, spectral data and literature references were polymethoxylated avones, C‐glycosylated avones, O‐glycosylated avones, O‐glycosylated avanones, avonols and phenolic acids and their derivatives. The ethyl acetate fraction which has been shown in previous work to possess the best radical scavenging activity among the others was found to contain C‐glycosylated avones, polymethoxylated avones, O‐glycosylated avones, O‐glycosylated avanones, two phenolic acid derivatives and two unknown compounds, all in low concentrations. The group of C‐glycosylated avones was reported for the rst time in the peel of Navel sweet orange. The C‐glycosylated avones found according to their spectral characteristics and literature were 6‐C‐ β ‐glucosyldiosmin, 6,8‐di‐C‐glucopyranosylapigenin, 6,8‐di‐C‐ β ‐glucosyldiosmin and two unknown. The results suggest that the ethyl acetate fraction of navel Citrus sinensis peel consists of signicant antioxidant compounds and can be used as a food additive of natural origin or a pharmaceutical supplement using as a source of peel the byproducts of the orange juice industry. Copyright © 2004 John Wiley & Sons, Ltd.