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Preparative isolation of ganoderic acid S, ganoderic acid T and ganoderol B from Ganoderma lucidum mycelia by high‐speed counter‐current chromatography
Author(s) -
Feng Na,
Wei Yutian,
Feng Jie,
Tang Qingjiu,
Zhang Zhong,
Zhang Jingsong,
Han Wei
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4283
Subject(s) - chemistry , chromatography , countercurrent chromatography , methanol , ganoderma , ethyl acetate , petroleum ether , ethanol , hexane , solvent , column chromatography , extraction (chemistry) , ganoderma lucidum , organic chemistry , food science
Abstract Ganoderic acid S, ganoderic acid T and ganoderal B are the main bioactive triterpenes of Ganoderma lucidum . In this study, mycelia of G. lucidum were obtained by two‐stage fermentation and then extracted by ethanol and petroleum ether sequentially to obtain crude triterpenes. The crude sample was further purified by recycling high‐speed counter‐current chromatography with n‐ hexane–ethyl acetate–methanol–water (7:12:11:5, v /v/ v /v) as the optimized two‐phase solvent system. A 16.4 mg aliquot of ganoderol B with a purity of 90.4% was separated from 300 mg of the crude sample in a single run. After employing the recycling elution mode of HSCCC with n‐ hexane–ethyl acetate–methanol–water (6:10:8:4.5, v /v/ v /v) for five cycles, 25.7 mg ganoderic acid T and 3.7 mg ganoderic acid S with purities of 97.8 and 83.0%, respectively, were obtained. The purities of three compounds were determined by high‐performance liquid chromatography and their chemical structures were identified by NMR and MS data.