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Determination of armepavine in mouse blood by UPLC‐MS/MS and its application to pharmacokinetic study
Author(s) -
Geng Peiwu,
Luo Jun,
Weng Ziwei,
Fan Zhehua,
Zhang Bin,
Ma Jianshe,
Wang Xianqin,
Zhang Meiling
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4273
Subject(s) - chromatography , formic acid , chemistry , ammonium acetate , selected reaction monitoring , pharmacokinetics , high performance liquid chromatography , calibration curve , tandem mass spectrometry , bioavailability , detection limit , mass spectrometry , gradient elution , matrix (chemical analysis) , quantitative analysis (chemistry) , acetonitrile , liquid chromatography–mass spectrometry , pharmacology , medicine
The purpose of this study was to develop an ultra‐performance liquid chromatography with tandem mass spectrometry (UPLC‐MS/MS) method to determine armepavine in mouse blood. Nuciferine was used as internal standard. Chromatographic separation was performed on a UPLC BEH (2.1 × 50 mm, 1.7 μm) column with a gradient elution of acetonitrile and 10 mmol/L ammonium acetate solution (containing 0.1% formic acid). The quantitative analysis was conducted in multiple reaction monitoring mode with m / z 314.1 → 106.9 for armepavine and m / z 296.2 → 265.1 for nuciferine. Calibration curves were linear ( r > 0.995) over the concentration range 1–1000 ng/mL in mouse blood with a lowest limit of quantitation of 1 ng/mL. The intra‐ and inter‐day precisions of armepavine in mouse were < 13.5 and 10.8%, respectively. The accuracy ranged between 86.8 and 103.3%. Meanwhile, the average recovery was >70.7% and the matrix effect was within the range 109.5–113.7%. All of the obtained data confirmed the satisfactory sensitivity and selectivity of the developed method which was then successfully applied to evaluate the pharmacokinetic behavior of armepavine in mouse for the first time. The bioavailability of armepavine in mouse was calculated to be 11.3%.