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Simultaneous determination of multicomponent of acetylkitasamycin and kitasamycin by LC–MS/MS in swine plasma and its application in a pharmacokinetic study
Author(s) -
Pan Yuanhu,
Zhang Heying,
Xi Chenglong,
Huang Lingli,
Xie Shuyu,
Chen Dongmei,
Tao Yanfei,
Liu Zhenli,
Yuan Zonghui
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4268
Subject(s) - chromatography , pharmacokinetics , chemistry , ammonium acetate , electrospray ionization , analyte , selected reaction monitoring , elution , mass spectrometry , high performance liquid chromatography , tandem mass spectrometry , pharmacology , medicine
A simple and reliable LC–MS/MS method was established for simultaneous determination of 12 components from acetylkitasamycin and kitasamycin in swine plasma. The analytes were separated on a Shim‐pack VP‐ODS column with a 25 min gradient elution using 5 mmol/L ammonium acetate and acetonitrile as the mobile phase at a flow rate of 0.2 mL/min. Identification and quantification were accomplished by electrospray ionization) in positive mode using multiple reaction monitoring. The limits of quantitation of acetylkitasamycin A 1 A 3 , A 13 and kitasamycin A 3 , A 13 were 3 μg/L, and that of the other eight components was 5 μg/L. The mean recoveries of kitasamycin and acetylkitasamycin ranged from 85.3 to 103.5%. The developed method was successfully applied to a pharmacokinetic study in swine after intravenous (i.v.) and oral (p.o.) administration of acetylkitasamycin. The result showed that the plasma concentrations of acetylkitsamycin components were much higher than that of kitasamycin in swine after i.v. and p.o., in which acetylkitsamycin A 4 A 5 was the highest component at each time point.