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Inhibitory effects of Danhong Injection and its major constituents on human cytochrome P450 enzymes in vitro
Author(s) -
Ye LinHu,
Zhao XinQian,
Kong LingTi,
Wang LiSha,
Tao Xue,
Wu Hui,
He Mei,
Chang Qi
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4250
Subject(s) - chemistry , cyp2e1 , cyp2c9 , cyp3a4 , pharmacology , cyp2d6 , cyp2c19 , in vitro , cytochrome p450 , rosmarinic acid , chromatography , microsome , enzyme , biochemistry , antioxidant , medicine
Danhong Injection (DHI) as a Chinese patent medicine is mainly used to treat ischemic encephalopathy and coronary heart disease in combination with other chemotherapy. However, the information on DHI's potential drug interactions is limited. The goal of this work was to examine the potential P450‐mediated metabolism drug interaction arising from DHI and its active components. The results showed that DHI inhibited CYP2C19, CYP2D6, CYP3A4, CYP2E1 and CYP2C9 with IC 50 values of 1.26, 1.42, 1.63, 1.10 and 1.67% ( v /v), respectively. Danshensu and rosmarinic acid inhibited CYP2E1 and CYP2C9 with IC 50 values of 36.63 and 75.76 μ m , and 34.42 and 76.89 μ m , respectively. Salvianolic acid A and B inhibited CYP2D6, CYP2E1 and CYP2C9 with IC 50 values of 33.79, 21.64 and 31.94 μ m , and 45.47, 13.52 and 24.15 μ m , respectively. The study provides some useful information for safe and effective use of DHI in clinical practice.