A validated method for analysis of chromium picolinate in nutraceuticals by reversed phase high performance liquid chromatography | Zendy

Koll Michael | Zendy; Hoenen Hubertus | Zendy; AboulEnein Hassan Y. | Zendy

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A validated method for analysis of chromium picolinate in nutraceuticals by reversed phase high performance liquid chromatography

Author(s) -

Koll Michael,

Hoenen Hubertus,

AboulEnein Hassan Y.

Publication year - 2005

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.424

Subject(s) - chemistry , chromatography , detection limit , chromium , high performance liquid chromatography , acetonitrile , linear range , phase (matter) , reversed phase chromatography , linear relationship , analytical chemistry (journal) , organic chemistry , statistics , mathematics

A validated high performance liquid chromatographic method was developed for the determination of chromium picolinate in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed‐phase Supelcosil LC‐18 (250 × 4.6 mm, 5 µm) column. The mobile phase consisted of acetonitrile:water (40:60 v/v) at a ow rate of 0.8 mL/min. The UV‐detector was set at 264 nm. The developed method showed a good linear relationship in the concentration range from 0.125 to 12.5 µg/mL with a correlation coefcient from 0.999. The limit of detection and limit of quantication were 0.091 and 0.181 µg/mL, respectively. Copyright © 2004 John Wiley & Sons, Ltd.

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