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A liquid chromatography–mass spectrometry method for the quantification of both etoricoxib and valdecoxib in human plasma
Author(s) -
Werner Ulrike,
Werner Dierk,
Hinz Burkhard,
Lambrecht Christoph,
Brune Kay
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.423
Subject(s) - valdecoxib , etoricoxib , chemistry , chromatography , mass spectrometry , selected reaction monitoring , pharmacokinetics , tandem mass spectrometry , analytical chemistry (journal) , cyclooxygenase , pharmacology , organic chemistry , medicine , rofecoxib , enzyme
Abstract A practicable and selective liquid chromatography–mass spectrometry assay for the determination of two cyclooxygenase‐2 inhibitors, etoricoxib and valdecoxib, in human plasma is presented. The analytical technique is based on reversed‐phase high‐performance liquid chromatography (HPLC) coupled to atmospheric pressure chemical ionisation (APCI) mass spectrometry (Finnigan Mat LCQ ion trap). Mass analysis was performed in the positive ion mode. The ion trap was operated in the tandem MS mode (MS 2 ) and the transitions of etoricoxib ( m / z 359.2 → 280.3) and valdecoxib ( m / z 315.1 → 235.1) were followed by selected reaction monitoring. Retention times of etoricoxib and valdecoxib were 1.05 and 1.08 min, respectively. The method was validated over a linear range 10–2500 and 5–1000 µg/L using the other substrate as internal standard. After validation, the method was used to study the pharmacokinetic prole of etoricoxib or valdecoxib in a healthy volunteer after administration of a single oral dose (valdecoxib, 20 mg; etoricoxib, 90 mg). The presented method was sufcient to cover more than 90% of the area under the plasma concentration time curve. Copyright © 2004 John Wiley & Sons, Ltd.