Premium
Simultaneous determination of isochamaejasmin, neochamaejasmin A and aphnoretinin rat plasma by UPLC‐MS/MS and its application to a pharmacokinetic study of Stellera chamaejasme L. extract
Author(s) -
Wang Ludi,
Yang Wei,
Wu Siyang,
Wang Shuyao,
Kang Chen,
Ma Xiaoli,
Li Yingfei,
Li Chuan
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4162
Subject(s) - chemistry , formic acid , chromatography , electrospray ionization , high performance liquid chromatography , pharmacokinetics , methanol , analyte , aqueous solution , electrospray , analytical chemistry (journal) , mass spectrometry , pharmacology , medicine , organic chemistry
Isochamaejasmin, neochamaejasmin A and daphnoretin derived from Stellera chamaejasme L. are important because of their reported anticancer properties. In this study, a sensitive UPLC‐MS/MS method for the determination of isochamaejasmin, neochamaejasmin A and daphnoretin in rat plasma was developed. The analyte and IS were separated on an Acquity UPLC HSS T3 column (100 × 2.1 mm, 1.8 μm) using gradient elution with the mobile phase of aqueous solution (methanol–water, 1:99, v/v, containing 1 m m formic acid) and organic solution (methanol–water, 99:1, v/v, containing 1 m m formic acid) at a flow rate of 0.3 mL/min. Multiple reaction monitoring mode with negative electrospray ionization interface was carried out to detect the components. The method was validated in terms of specificity, linearity, accuracy, precision, stability, etc. Excellent linear behavior was observed over the certain concentration ranges with the correlation coefficient values >0.99. Intra‐ and inter‐day precisions (RSD) were <6.7% and accuracy (RE) ranged from −7.0 to 12.0%. The validated method was successfully applied to investigate the pharmacokinetics of three chemical ingredients after oral administration of S. chamaejasme L. extract to rats.