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Metabolic studies on the total phenolic acids from the roots of Salvia miltiorrhiza in rats
Author(s) -
Zhang Jinlan,
He Yun,
Cui Ming,
Li Lie,
Yu Hailan,
Zhang Guifeng,
Guo Dean
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.415
Subject(s) - salvia miltiorrhiza , chemistry , ferulic acid , caffeic acid , chromatography , high performance liquid chromatography , phenolic acid , extraction (chemistry) , metabolic pathway , metabolism , biochemistry , antioxidant , traditional chinese medicine , medicine , alternative medicine , pathology
Phenolic acids are the main active constituents of Salvia miltiorrhiza Bunge. The metabolism of total phenolic acids from the roots of Salvia miltiorrhiza in rats was investigated. A sample preparation method combining the solid‐phase extraction with liquid–liquid extraction was established to separate metabolites from the biological matrix. HPLC‐UV and HPLC‐MS methods were employed to analyze the metabolites. Five metabolites (M1–M5) were identied by HPLC‐MS analysis and comparison with those of the reference standards. The ve metabolites were characterized as danshensu (M1), caffeic acid (M2), ferulic acid (M3), isoferulic acid (M4) and methylized ferulic acid (M5), respectively. The possible metabolic pathway of the phenolic acids is proposed. Copyright © 2004 John Wiley & Sons, Ltd.