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Simultaneous separation and analysis of camptothecin alkaloids in real samples by large‐volume sample stacking in capillary electrophoresis
Author(s) -
Chen Meng,
Huang Yayun,
Xu Liying,
Zhang Hongfen,
Zhang Guangbin,
Chen Anjia
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4125
Subject(s) - capillary electrophoresis , chemistry , chromatography , analyte , stacking , analytical chemistry (journal) , capillary action , camptothecin , linearity , volume (thermodynamics) , materials science , physics , organic chemistry , quantum mechanics , composite material
Abstract Large‐volume sample stacking (LVSS) is commonly used as an effective online preconcentration method in capillary zone electrophoresis (CZE). In this paper, the method LVSS combined with CZE has been proposed to analyze camptothecin alkaloids. Optimum separation can be achieved in the following conditions: pH 9.0; 25m m borate buffer containing 20 m m sulfobutylether‐ β ‐cyclodextrin and 20 m m ionic liquid 1‐ethyl‐3‐methyllimidazole l ‐lactate; applied voltage 20 kV; and capillary temperature 25 °C. The LVSS was optimized as hydrodynamic injection 4 s at 5.0 psi and the polarity switching time was 0.17 min. Under the above conditions, the analytes could be separated completely in <20 min and the detector response was increased compared with conventional hydrodynamic injection. The limits of detection were between 0.20 and 0.78 μg/L. A good linearity was obtained with correlation coefficients from 0.9991 to 0.9997. The recoveries ranged from 97.72 to 103.2% and the results demonstrated excellent accuracy. In terms of the migration time and peak area, the experiment was reproducible. The experimental results indicated that baseline separation can be obtained and this method is suitable for the quantitative determination of camptothecin alkaloids in real samples.