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A target and nontarget strategy for identification or characterization of the chemical ingredients in Chinese herb preparation Shuang‐Huang‐Lian oral liquid by ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry
Author(s) -
Zhang FengXiang,
Li Min,
Yao ZhiHong,
Li Chang,
Qiao LiRui,
Shen XiuYu,
Yu Kate,
Dai Yi,
Yao XinSheng
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4110
Subject(s) - phenylethanoid , quadrupole time of flight , chromatography , chemistry , glycoside , mass spectrometry , high performance liquid chromatography , electrospray ionization , organic chemistry
A target and nontarget strategy based on in‐house chemical components library was developed for rapid and comprehensive analysis of complicated components from traditional Chinese medicine preparation Shuang‐Huang‐Lian oral liquid. The sample was analyzed by ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry using generic acquisition parameters. Automated detection and data filtering were performed on the UNIFI™ software and the detected peaks were evaluated against an in‐house library. As a result, a total of 170 chemical components (110 target compounds and 60 nontarget ones) were identified or tentatively characterized, including 54 flavonoids, 30 phenylethanoid glycosides, 16 iridoid glycosides, 14 lignans, 32 organic acids, 19 triterpenoid saponins and five other types of compounds. Among them, 44 compounds were further confirmed by comparison with reference standards. It was demonstrated that this systematical approach could be successfully applied for rapid identification of multiple compounds in traditional Chinese medicine and its preparations. Furthermore, this work established the foundation for the further investigation on the metabolic fates of multiple ingredients in Shuang‐Huang‐Lian oral liquid.