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Determination of enrofloxacin and its primary metabolite, ciprofloxacin, in pig tissues. Application to residue studies
Author(s) -
Garcia M. A.,
Solans C.,
Calvo A.,
Hernandez E.,
Rey R.,
Bregante M. A.,
Puig M.
Publication year - 2005
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.411
Subject(s) - chemistry , chromatography , elution , metabolite , residue (chemistry) , solid phase extraction , phosphate buffered saline , high performance liquid chromatography , acetonitrile , extraction (chemistry) , biochemistry
A simple and sensitive HPLC method has been developed for the simultaneous determination of enrooxacin (ENR) and ciprooxacin (CIP) in pig tissue using dioxacin (DIF) as internal standard. Tissue sample preparations were carried out by adding phosphate buffer (pH 7.4, 0.1 m ), followed by extraction with trichloromethane. Fluoroquinolones were separated on a reversed‐phase column and eluted with aqueous buffer solution‐acetonitrile (80:20, v/v). The concentrations of CIP, ENR and DIF eluted from the column, with retention times of 2.20, 2.73 and 4.38 min, respectively, were monitored by uorescence detection at λ ex 276 and λ em 442 nm. The detection and quantitation limit were 8 and 25 ng/g, respectively, for both compounds. Standard curves were linearly related to concentration in the range 25–400 ng/g. The consequences of the introduction of minor reasonable variations (ruggedness studies) have also been analysed. Finally, the measurement of the tissue levels of ENR and CIP in the pig tissues after oral administration conrmed the utility of the proposed method. Copyright © 2004 John Wiley & Sons, Ltd.