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Simple and extractionless high‐performance liquid chromatographic determination of rosiglitazone in human plasma and application to pharmacokinetics in humans
Author(s) -
Kim KyoungAh,
Park JiYoung
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.410
Subject(s) - chemistry , chromatography , ammonium acetate , perchloric acid , high performance liquid chromatography , pharmacokinetics , rosiglitazone , acetonitrile , plasma , human plasma , pharmacology , receptor , medicine , biochemistry , physics , organic chemistry , quantum mechanics
A simple and extractionless HPLC method using uorescence detection was developed for the determination of rosiglitazone in human plasma. After deproteinization using perchloric acid the plasma samples were directly injected onto the HPLC system. The mobile phase was composed of acetonitrile (52%) and 20 m m ammonium acetate (48%, pH 7.5), and analysis was run at a ow rate of 0.2 mL/min with the detector operating at 247 nm for excitation wavelength and at 367 nm for emission wavelength, respectively. The method has a mean recovery of 97%, while the intra‐day and inter‐day precisions were all less than 7%. This method is simple, specic, sensitive and requires only a small plasma volume with short analytical time, and is suitable for the determination of plasma rosiglitazone in routine measurements for pharmacokinetic studies. Copyright © 2004 John Wiley & Sons, Ltd.