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Simultaneous determination of baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re of Banxia xiexin decoction in rat plasma by LC–MS/MS and its application to a pharmacokinetic study
Author(s) -
Wang Ying,
Zhang Yifan,
Xiao Juan,
Xu Ranchi,
Wang Qiangli,
Wang Xinhong
Publication year - 2018
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4083
Subject(s) - chemistry , coptisine , chromatography , baicalin , baicalein , scutellarin , decoction , paeoniflorin , pharmacokinetics , berberine , wogonin , selected reaction monitoring , formic acid , mass spectrometry , tandem mass spectrometry , palmatine , high performance liquid chromatography , pharmacology , scutellaria baicalensis , traditional chinese medicine , traditional medicine , biochemistry , medicine , alternative medicine , pathology
Abstract A rapid and high‐sensitivity liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for simultaneous determination of nine active constituents (baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re) in rat plasma after oral administration of Banxia xiexin decoction (BXD). Biological samples were processed wtih acetone–ethyl acetate (4:1, v /v). The mobile phase consisted of methanol and water (containing 0.1% formic acid) with gradient elution at a flow rate of 0.3 mL/min. Detection was performed on a triple quadrupole mass spectrometer using positive ion and negative ESI in the multiple reaction monitoring mode. The calibration curves for all analytes had good linearity ( r > 0.9933). The mean recovery of all the nine active ingredients was >75.2%, and the intra‐ and inter‐day precisions were within 12.0%; the accuracy was between 87.4 and 110.4%. This method was successfully applied to a pharmacokinetic study after administration of BXD. The results of the pharmacokinetic study might be helpful for BXD reasonable clinical application and further studies on mechanism.