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A more sensitive MS detector does not obviously lead to a more sensitive assay: experiences with ES‐285
Author(s) -
Stokvis Ellen,
Rosing Hilde,
LópezLázaro Luis,
Schellens Jan H. M.,
Beijnen Jos H.
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.404
Subject(s) - chemistry , chromatography , human plasma , mass spectrometry , detection limit , detector , tandem , tandem mass spectrometry , liquid chromatography–mass spectrometry , quantitative analysis (chemistry) , materials science , electrical engineering , composite material , engineering
In this paper the transfer of an existing method for the quantitative determination of the anticancer agent ES‐285 in human plasma using liquid chromatography tandem mass spectrometry on an API 365 to an API 3000 mass spectrometer is described. The transfer appeared not to be straightforward. Problems arose resulting from carry‐over and interferences. In addition, due to the expansion of the calibration range, data ought to be weighted with a different factor to increase the accuracy of the lower concentrations. After nding appropriate solutions for these problems, the lower limit of quantitation could be lowered from 10 to 1 ng/mL for ES‐285 in human plasma. The usefulness and necessity of the modied assay was demonstrated by analysis of plasma samples from a patient receiving a low dosage of the drug. Copyright © 2004 John Wiley & Sons, Ltd.