Premium
Quantification of β ‐eudesmol in rat plasma using LC–MS/MS and its application to a pharmacokinetic study
Author(s) -
Jiang Ligang,
Zhang Chunyang,
Li Haiping
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4023
Subject(s) - chemistry , chromatography , formic acid , pharmacokinetics , analyte , selected reaction monitoring , extraction (chemistry) , acetonitrile , elution , ether , mass spectrometry , tandem mass spectrometry , pharmacology , medicine , organic chemistry
Abstract A sensitive and specific LC–MS/MS assay for determination of β ‐eudesmol in rat plasma was developed and validated. After liquid–liquid extraction with ethyl ether, the analyte and IS were separated on a Capcell Pak C 18 column (50 × 2.0 mm, 5 μm) by isocratic elution with acetonitrile—water–formic acid (77.5:22.5:0.1, v /v/v) as the mobile phase at a flow rate of 0.4 mL/min. An ESI source was applied and operated in positive ion mode; a selected reaction monitoring scan was used for quantification by monitoring the precursor–product ion transitions of m/z 245.1 → 163.1 for β ‐eudesmol and m/z 273.4 → 81.2 for IS. Good linearity was observed in the concentration range of 3–900 ng/mL for β ‐eudesmol in rat plasma. Intra‐ and inter‐day precision and accuracy were both within ±14.3%. This method was applied for pharmacokinetic studies after intravenous bolus of 2.0 mg/kg or intragastric administration of 50 mg/kg β ‐eudesmol in rats.