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Relationship between the UPLC‐Q‐TOF‐MS fingerprinted constituents from Daphne genkwa and their anti‐inflammatory, anti‐oxidant activities
Author(s) -
Du WenJuan,
Ji Jun,
Wang Ling,
Lan XinYi,
Li Jia,
Lei JunQiu,
He Xin,
Zhang ChunFeng,
Huang WenZhe,
Wang ZhenZhong,
Xiao Wei,
Wang ChongZhi,
Yuan ChunSu
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.4012
Subject(s) - fingerprint (computing) , chemistry , thymelaeaceae , chromatography , traditional medicine , dpph , chemical constituents , positive control , antioxidant , botany , artificial intelligence , biology , medicine , biochemistry , computer science
Daphne genkwa Sieb.et Zucc. is a well‐known medicinal plant. This study was designed to apply the ultra‐high performance liquid chromatography system to establish a quality control method for D. genkwa. Data revealed that there were 15 common peaks in 10 batches of D. genkwa Sieb. Et Zucc. (Thymelaeaceae) from different provinces of China. On this basis, the fingerprint chromatogram was established to provide references for quality control. Afterwards, the chemical constitutions of these common peaks were analyzed using the UPLC‐Q‐TOF‐MS system and nine of them were identified. In addition, LPS‐stimulated RAW264.7 murine macrophages and DPPH assay were used to study the anti‐inflammatory and anti‐oxidation effects of D. genkwa . Then the fingerprint–efficacy relationships between UPLC fingerprints and pharmacodynamic data were studied with canonical correlation analysis. Analysis results indicated that the anti‐inflammatory and anti‐oxidation effects differed among the 10 D. genkwa samples owing to their inherent differences of chemical compositions. Taken together, this research established a fingerprint–efficacy relationship model of D. genkwa plant by combining the UPLC analytic technique and pharmacological research, which provided references for the detection of the principal components of traditional Chinese medicine on bioactivity.