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Identification and determination of effective components in Euphrasia regelii by capillary zone electrophoresis
Author(s) -
Shuya Cui,
Shengda Qi,
Xingguo Chen,
Zhide Hu
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.401
Subject(s) - chemistry , capillary electrophoresis , chromatography , analyte , electrophoresis , electrolyte , cinnamic acid , ferulic acid , analytical chemistry (journal) , biochemistry , electrode
A capillary zone electrophoresis (CZE) method has been developed for simultaneous determination of eukovoside, cinnamic acid and ferulic acid in Euphrasia regelii for the rst time. The electrophoresis buffer was 20 mmol/L sodium borate containing 10% (v/v) methanol (pH 8.50). The effects of concentration of borate and electrolyte pH on electrophoretic behavior and separation were studied. Regression equations revealed linear relationships (correlation coefcients 0.9995–0.9998) between the peak area of each analyte and the concentration. The levels of analytes in the different parts of Euphrasia regelii were easily determined with recoveries ranging from 95.5 to 104.2%. Copyright © 2004 John Wiley & Sons, Ltd.