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HPLC determination of four active saponins from Panax notoginseng in rat serum and its application to pharmacokinetic studies
Author(s) -
Li Lie,
Sheng Yuxin,
Zhang Jinlan,
Wang Chuanshe,
Guo Dean
Publication year - 2004
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.400
Subject(s) - panax notoginseng , chemistry , chromatography , pharmacokinetics , high performance liquid chromatography , traditional medicine , pharmacology , medicine , alternative medicine , pathology
Four main active saponins (ginsenosides Rg 1 , Rb 1 , Rd and notoginsenoside R 1 ) in Panax notoginseng in rat serum after oral and intravenous administration of total saponins of P. notoginseng (PNS) to rats were determined using a simple and sensitive high‐performance chromatographic method. The serum samples were pretreated with solid‐phase extraction before analysis. The calibration curves for the four saponins were linear in the given concentration ranges. The intra‐day and inter‐day assay coefcients in serum were less than 10.0% and the recoveries of the method were higher than 80.0% in the high, middle and low concentrations. This method was applied to study the pharmacokinetics following oral and intravenous administration of PNS. Copyright © 2004 John Wiley & Sons, Ltd.