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Analysis of toldimfos in porcine muscle and bovine milk using liquid chromatography–triple quadrupole mass spectrometry
Author(s) -
Zhang Dan,
Park JinA,
Abd ElAty A. M.,
Kim SeongKwan,
Cho SangHyun,
Zheng Weijia,
Choi Jeongmin,
Shim JaeHan,
Chang ByungJoon,
Kim JinSuk,
Shin HoChul
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3996
Subject(s) - chemistry , ammonium formate , chromatography , ammonium acetate , mass spectrometry , detection limit , acetonitrile , triple quadrupole mass spectrometer , liquid chromatography–mass spectrometry , extraction (chemistry) , tandem mass spectrometry , sodium formate , high performance liquid chromatography , selected reaction monitoring
Abstract An analytical method was developed for the detection of toldimfos sodium residues in porcine muscle and bovine milk using liquid chromatography–triple quadrupole tandem mass spectrometry (LC–MS/MS) analysis. The drug was extracted from muscle and milk using 10 m m ammonium formate in acetonitrile and then purified using n ‐hexane. The drug was well separated on a Luna C 18 column using a mixture of 10 m m ammonium formate in ultrapure water (A) and acetonitrile (B) as the mobile phase. Good linearity was achieved over the tested concentration range (0.005–0.03 mg/kg) in matrix‐matched standard calibration. The determination coefficients ( R 2 ) were 0.9942 and 0.9898 for muscle and milk, respectively. Fortified porcine muscle and bovine milk contained concentrations equivalent to and twice the limit of quantification (0.005 mg/kg) yielded recoveries in the range of 75.58–89.74% and relative standard deviations of ≤8.87%. Samples collected from large markets located in Seoul, Republic of Korea, tested negative for toldimfos sodium residue. In conclusion, ammonium formate in acetonitrile can effectively extract toldimfos sodium from porcine muscle and bovine milk without solid‐phase extraction, which is usually required for cleanup before analysis. This method can be applied for the routine analysis of toldimfos in foods of animal origins.