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LC/QTOF profile and preliminary stability studies of an enriched flavonoid fraction of Cecropia pachystachya Trécul leaves with potential antidepressant‐like activity
Author(s) -
Ortmann Caroline Flach,
Abelaira Helena Mendes,
Réus Gislaine Zilli,
Ignácio Zuleide Maria,
Chaves Vitor Clasen,
Santos Talitha Caldas,
Carvalho Pâmela,
Carlessi Anelise Scussel,
Bruchchen Livia,
Danielski Lucineia G.,
Cardoso Simone Gonçalves,
Campos Angela Machado,
Petronilho Fabricia,
Rebelo Joyce,
Santos Morais Meline Oliveira,
Vuolo Francieli,
DalPizzol Felipe,
Streck Emilio Luiz,
Quevedo João,
Reginatto Flávio Henrique
Publication year - 2017
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.3982
Subject(s) - orientin , chemistry , vitexin , isovitexin , isoorientin , flavonoid , behavioural despair test , antioxidant , antidepressant , biochemistry , neuroscience , biology , hippocampus
Abstract There is increasing interest in natural antioxidants that are candidates for the prevention of brain damage occurring in major depressive disorders. Cecropia pachystachya is a tropical tree species of Central and South America and a rich source of polyphenols, particularly flavonoids. The aim of this study was to characterize the flavonoid profile of an enriched flavonoid fraction of C. pachystachya (EFF‐ Cp ) and evaluate the antidepressant‐like effects of its acute administration in behavior, cytokine levels, oxidative stress and energy metabolism parameters . The EFF‐ Cp chemical characterization was performed by HPLC/DAD and LC/QTOF. The antidepressant‐like effects were performed by the forced swimming test, splash test and open field test. EFF‐ Cp revealed 15 flavonoids, including seven new glycosyl flavonoids for C. pachystachya . Quantitatively, EFF‐ Cp showed isoorientin (43.46 mg/g), orientin (23.42 mg/g) and isovitexin (17.45 mg/g) as major C ‐glycosyl flavonoids. In addition, EFF‐ Cp at doses 50 and 100 mg/kg reduced the immobility time in the forced swimming test, without changing the locomotor activity and grooming time. In addition, EFF‐ Cp was able to prevent the oxidative damage in some brain areas. In conclusion, the results of this study suggest that EFF‐ Cp exerts antidepressant‐like effects with its antioxidant properties.